pgsB-618细胞
ATCC Number： CRL-2241™
数量： 大量
相关**： 其他**
细胞形态： 上皮样
生长状态： 贴壁生长
器官来源： 卵巢
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 仓鼠
Designations: pgsB-618
Depositors: JD Esko
pgsB-618细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Cricetulus griseus
Morphology: epithelial
Source: Organ: ovary
Disease: galactosyltransferase I deficient
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: NOTE: pgsB-618细胞This line is available subject to the following: 1.) The CHO cell mutant was deposited in the ATCC by Dr. Jeffrey D. Esko and is provided for research purposes only as a service to the research community. They are provided without warranty or merchantability of fitness for a particular purpose or any other warranty, express or implied. The cells are provided with the understanding that they will not be used for commercial purposes. 2.) All products derived from the cells or genetically altered forms of the cells cannot be commercialized without express permission from the University of Alabama at Birmingham. Commercial interests are the exclusive property of the University of Alabama at Birmingham. 3.) Any proposed commercial uses of these cells must first be negotiated with the Research Foundation, University of Alabama at Birmingham, Birmingham, AL 35294. Telephone: (205) 934-9911. 4.) In all papers reporting any use of these cells or derived products, a direct reference will be made to the original publication listed above.
Gender: female
Comments: PgsB-618 is a Chinese hamster ovary cell mutant deficient in galactosyltransferase I (UDP-D-galactose:xylose-1,4-D-galactosyl- transferase).
The cell line was derived from CHO-K1 cells (see ATCC CCL-61) treated with mutagen (ethylmethanesulfonate) and screened for mutants defective in proteoglycan synthesis.
PgsB-618 cells do not produce glycosaminoglycans.
Propagation: pgsB-618细胞ATCC complete growth medium: Ham's F12 medium, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Rinse the cell sheet with fresh 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the culture to sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 23505: Esko JD, et al. Tumor formation dependent on proteoglycan biosynthesis. Science 241: 1092-1096, 1988. PubMed: 3137658
23546: Esko JD, et al. Inhibition of chondroitin and heparan sulfate biosynthesis in Chinese hamster ovary cell mutants defective in galactosyltransferase I. J. Biol. Chem. 262: 12189-12195, 1987. pgsB-618细胞PubMed: 2957376
