JeKo-1细胞
年限： 78
器官来源： 外周血
是否是肿瘤细胞： 0
物种来源： 人
生长状态： 悬浮生长
数量： 大量
ATCC Number： CRL-3006™
细胞形态： **样
运输方式： 冻存运输
组织来源： Mantle Cell Lymphoma
Designations: JeKo-1
JeKo-1细胞Depositors: Elias Campo
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Characterization Data
Source: Organ: peripheral blood
Tissue: Mantle Cell Lymphoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: CD3-, CD5+, CD10+, CD19+ (verified at ATCC )
JeKo-1细胞DNA Profile (STR): Amelogenin:X
CSF1PO:9,12
D13S317:8,9
D16S539:12
D5S818:10,13
D7S820:10,11
THO1:7
TPOX:8
vWA: 14
Age: 78
Gender: female
Comments: The JeKo-1 Mantle cell lymphoma (MCL) cell line was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukemic conversion. JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype. The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. [PubMed: 9753063]
Propagation:JeKo-1细胞 ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Subculturing: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10(4) to 4 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 2.5 X 10(6) cells/ml.
Preservation: Freeze medium: 30% RPMI; 60% FBS; 10% DMSO
Storage temperature: liquid nitrogen vapor phase
References: 92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365
16172573: Camps, J., et al. JeKo-1细胞Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697
16172575: Jeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063
