2Hx-2细胞
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
细胞形态： **样
**类型： IgG2a
ATCC Number： HB-8117™
运输方式： 冻存运输
Designations: 2Hx-2
Depositors: Massachusetts Institute of Technology
2Hx-2细胞Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against adenovirus group specific antigen
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Tumorigenic: Yes
Comments: Animals were immunized with Hela cells that were infected with adenovirus 2.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
2Hx-2细胞The antibody reacts with adenoviruses of human subgroups A through E as well as with simian, bovine, canine and swine adenoviruses.
It does not react with avian adenoviruses.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. 2Hx-2细胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1197: Cepko CL, et al. Immunoprecipitation with two-dimensional pools as a hybridoma screening technique: Production and characterization of monoclonal antibodies against adenovirus 2 proteins. Virology 110: 385-401, 1981. PubMed: 7222481
3554: Cepko CL, et al. Production and use of monoclonal antibodies against adenoviruses. US Patent 4,487,829 dated Dec 11 1984
22704: Cepko CL, et al. Adenovirus hexon monoclonal antibody that is group specific and potentially useful as a diagnostic reagent. J. Clin. Microbiol. 17: 360-364, 1983. 2Hx-2细胞PubMed: 6339552
