MG38细胞
器官来源： 脾
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
ATCC Number： CRL-2640™
运输方式： 冻存运输
生长状态： 悬浮生长
细胞形态： **样
**类型： IgG2b
Designations: MG38
MG38细胞Depositors: RM Steinman
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Organ: spleen
Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against the human DEC-205 antigen (human dendritic cell antigen)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. MG38细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: DEC-205-/- knock-out mice were immunized with purified protein from human DEC-205 cells. [53324]
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The antibody labels mature monocyte-derived dendritic cells but shows weak or no labeling of other peripheral blood mononuclear cells. [53324]
In tissue sections, MG38 identifies DEC-205 on thymic cortical epithelium and dendritic cells in the thymic medulla and tonsillar T cell areas. [53324]
The antibody reacts with DEC-205 in tissue sections from both humans and rhesus macaque monkeys.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp(5) viable cells/ml. MG38细胞Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Culture medium with an additional 5% fetal bovine serum, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 53324: Guo M, et al. MG38细胞A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells. Hum. Immunol. 61: 729-738, 2000. PubMed: 10980384
