5c8细胞
生长状态： 悬浮生长
数量： 大量
ATCC Number： HB-10916™
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： **样
**类型： mouse IgG2a
运输方式： 冻存运输
Designations: 5c8
Depositors: Trustees of Columbia University
Isotype: mouse IgG2a
5c8细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against T-B activating molecule T-BAM, ligand for CD40 (CD40L, CD154)
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Comments: Animals were immunized with D1.1 cells (see ATCC CRL-10915).
Spleen cells were fused with Sp2/0 myeloma cells.
5c8细胞The antibody recognizes an antigen present on T cells that is important to the T cell mediated activation of B cells.
The antigen is a ligand for the CD40 molecule on the B cell surface.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml.
Interval: Maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: 5c8细胞Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22085: Lederman S, et al. Murine monoclonal antibody (5c8) recognizes a human glycoprotein on the surface of T-lymphocytes, compositions containing same. US Patent 5,474,771 dated Dec 12 1995
72024: Lederman S, et al. Methods for treating autoimmune diseases with 5C8-specific antibodies. US Patent 6,592,868 dated Jul 15 2003
90139: .5c8细胞 Protein recognized by an antibody that specifically binds an epitope that is specifically bound by monoclonal antibody 5c8. U.S. Patent 6,793,924 dated Sep 21 2004
