SL-29细胞
生长状态： 贴壁生长
年限： embryo; 11 days gestation
器官来源： 胚胎
ATCC Number： CRL-1590™
细胞形态： 成纤维样
是否是肿瘤细胞： 0
物种来源： 其他
数量： 大量
运输方式： 冻存运输
Designations: SL-29
Depositors: RJ Hay
SL-29细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Gallus gallus
Morphology: fibroblast
Source: Organ: embryo
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Applications: transfection host
Age: embryo; 11 days gestation
Comments: SL-29细胞This cell line was developed by standard trypsinization of a decapitated 11-day SPAFAS leghorn embryo.
The cells have a doubling potential of 35 population doublings.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: tryptose phosphate broth to a final concentration of 5% and fetal bovine serum to a final concentration of 5%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: The tryptose phosphate broth should be prepared fresh every 2 weeks. Fresh glutamine is also very important for the growth of this line.
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: SL-29细胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37�C.
Subcultivation Ratio: An inoculation density of 5 X 10(4) viable cells per sq. cm. of flask or dish surface area is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 21863: . SL-29细胞Aging in cell and tissue cultures. New York: Plenum Press; 1970.
90269: Ramirez AD, et al. Antisporozoite antibodies with protective and nonprotective activities: in vitro and in vivo correlations using Plasmodium gallinaceum, an avian model. J. Eukaryot. Microbiol. 42: 705-708, 1995. PubMed: 8520586
