3.155细胞
数量： 大量
生长状态： 悬浮生长
细胞形态： **样
是否是肿瘤细胞： 0
物种来源： 大鼠
**类型： IgM
ATCC Number： TIB-211™
运输方式： 冻存运输
Designations: 3.155
Depositors: FW Fitch
Isotype: IgM
3.155细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against Lyt-2 (mouse cytotoxic T cell antigen, all alleles)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with CTL Clone L3 cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
3.155细胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. 3.155细胞Start cultures at 1 x 10 (5) cells/ml and maintain between 1 x 10 (5) and 1 x 10 (6) cells/ml.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
References: 984: Sarmiento M, et al. IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing lyt 2 antigen block T-cell mediated cytolysis in the absence of complement. J. Immunol. 125: 2665-2672, 1980. PubMed: 6159417
58011: Sarmiento M, et al. Cloned T lymphocytes and monoclonal antibodies as probes for cell surface molecules active in T cell-mediated cytolysis. Immunol. Rev. 68: 135-169, 1982. 3.155细胞PubMed: 6184304
