QCRL-1细胞
运输方式： 冻存运输
器官来源： 脾
生长状态： 悬浮生长
ATCC Number： HB-11765™
数量： 大量
细胞形态： **样
**类型： mouse IgG1 kappa
是否是肿瘤细胞： 0
物种来源： 小鼠
Designations: QCRL-1
Depositors: Queen's University at Kingston
Isotype: mouse IgG1 kappa
QCRL-1细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Organ: spleen
Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against a multidrug resistance protein (MRP)
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Comments: Animals were immunized with the multidrug resistant, small cell lung carcinoma, cell line H69AR (ATCC CRL-11351).
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
The antibody reacts with MRP (multidrug resistance protein). QCRL-1细胞MRP is a 190,000 integral membrane phosphoglycoprotein that is overexpressed in some drug-selected resistant cell lines and has been shown to cause multidrug resistance in transfected cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Attached cells can be scraped.
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can beestablished by centrifugation with subsequent resuspension in fresh medium at 2 to 5 X 10(5) viable cells/ml.
Interval: Maintain cultures at cell concentrations between 2 X 10(5) and 2 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: QCRL-1细胞Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 49655: Deeley RG, Cole SP. Antibodies to a multidrug resistance protein. US Patent 6,063,621 dated May 16 2000
49661: Hipfner DR, et al. Detection of the M(r) 190,000 multidrug resistance protein, MRP, with monoclonal antibodies. Cancer Res. 54: 5788-5792, 1994. QCRL-1细胞PubMed: 7954400
