Z-138细胞
细胞形态： **样
年限： 70 years
运输方式： 冻存运输
生长状态： 悬浮生长
数量： 大量
ATCC Number： CRL-3001™
相关**： 其他**
细胞类型： **母细胞
是否是肿瘤细胞： 0
物种来源： 人
Designations: Z-138
Depositors: Z Estrov
Z-138细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Characterization Data
Source: Cell Type: lymphoblast
Disease: mantle cell lymphoma
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Isolation: Isolation date: August 1990
Antigen Expression: CD3-, CD5-, CD10-, CD19+, CD20+, CD23+, FMC7- (verified at ATCC )
DNA Profile (STR): Z-138细胞Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,12
D16S539: 11
D5S818: 11,13
D7S820: 8
TPOX: 8
THO1: 6
vWA: 15,18
Cytogenetic Analysis: This is a hyperdiploid human cell line of male origin with a modal chromosome number of 49 and a 4% polyploidy rate. Most of the cells examined contained the t(11;14)(q13;q32) derivative chromosome common to Mantle Cell Lymphoma. Other derivative chromosomes present in most of the examined cells included: del(5)(p15), der(9)t(9;?), der(14)t(8;14)?, and add(17p). Overall, the karyology was consistent with published data.
Age: 70 years
Gender: male
Ethnicity: Z-138细胞Caucasian
Comments: The Z-138 cell line was derived from a patient who initially was diagnosed with chronic lymphocytic leukemia (CLL). About 2 years later, the neoplasm had transformed into an aggressive, mature B-cell acute lymphocytic leukemia (B-cell ALL). According to the criteria of the current World Health Organization lymphoma classification, this neoplasm is best classified as mantle cell lymphoma, with blastoid transformation present in the terminal phase of disease.
Z-138 cells have a complex karyotype, including the t(11;14)(q13;q32) abnormality, which is common in B-cell neoplasms.
These cells overexpress Cyclin D1.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) to 2 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 3 X 10(6) cells/ml.
Subcultivation ratio: A subcultivation ratio of 1:5 to 1:15 is recommended.
Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Preservation: Freeze medium: horse serum 90%; DMSO, 10%
liquid nitrogen vapor phase
Doubling Time: approximately 24 hours
Related Products: Z-138细胞Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2005
Recommended serum: ATCC 30-2040
Cell culture tested DMSO: ATCC 4-X
Erythrosin B vital stain solution: ATCC 30-2404
References: 92894: Tucker CA, et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk. Res. 30: 449-457, 2006. PubMed: 16183118
16173256: Medeiros LJ, et al. Z-138 cell line was derived from a patient with blastoid variant mantle cell lymphoma. Leuk. Res. 30(4):497-501, 2006. PubMed 16203034
16173257: Estrov Z, et al . Z-138: a new mature B-cell acute lymphoblastic leukemia cell line from a patient with transformed chronic lymphocytic leukemia. Leuk. Res.:22 (4):341-353, 1998. PubMed 9669839
