3B18细胞
生长状态： 混合型生长
是否是肿瘤细胞： 0
物种来源： 小鼠
运输方式： 冻存运输
数量： 大量
ATCC Number： HB-8654™
细胞形态： **样
**类型： IgG1
Designations: 3B18
3B18细胞Depositors: Salk Institute for Biological Studies
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: mixed: adherent and suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Cellular Products: immunoglobulin; monoclonal antibody; against a human mammary tumor cell cytoplasmic antigen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 3B18细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with MCF7 cells (ATCC HTB-22).
Spleen cells were fused with PAI myeloma cells.
The antibody cross-reacted with some cases of fibrocystic mammary disease and one of two colon carcinomas tested.
It did not react with normal tissue nor with 17 of 18 non-mammary malignancies tested.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 0.1 mM hypoxanthine, 0.02 mM thymidine, 0.01 mM 2-mercaptoethanol, and non-essential amino acids, 90%; horse serum, 10%
3B18细胞Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by transferring floating cells to additional flasks.
Attached cells may be subcultured by scraping.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
References: 3907: Dulbecco R, et al. 3B18细胞Monoclonal antibody specific for a mammary tumor cytoplasmic antigen. US Patent 4,628,032 dated Dec 9 1986
