3B11细胞
细胞类型： 其他细胞类型
是否是肿瘤细胞： 0
物种来源： 其他
运输方式： 冻存运输
3B11细胞器官来源： 外周血
ATCC Number： CRL-2757™
细胞形态： **样
生长状态： 悬浮生长
数量： 大量
Designations: 3B11
Depositors: NW Miller
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Ictalurus punctatus
3B11细胞Morphology: lymphoblast
Source: Organ: peripheral blood
Cell Type: lymphocyte B lymphoblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1994
Cytogenetic Analysis: diploid
Comments: 1G8 (ATCC CRL-2756), developed in 1992, and 3B11 (ATCC CRL-2757), developed in 1994, are immortal B cell lines developed by in vitro lipopolysaccharide (LPS) stimulation of peripheral blood from two different normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. They are reported to secrete moderate levels of IgM in culture [PubMed: 8133033].The 3B11 cells constitutively express telomerase [PubMed: 11687262].
Propagation: 3B11细胞ATCC complete growth medium: A 1:1 mixture of Leibovitz's L-15 medium with 2 mM L-glutamine, and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 87.5%; double distilled water, 10%; heat-inactivated Catfish serum, 2.5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 27.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml.
Interval: Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
References: 61261: Wilson M, et al. A novel chimeric Ig heavy chain from a teleost fish shares similarities to IgD. Proc. Natl. Acad. Sci. USA 94: 4593-4597, 1997. PubMed: 9114035
61262: Stuge TB, et al. Development and analysis of various clonal alloantigen-dependent cytotoxic cell lines from channel catfish. J. Immunol. 164: 2971-2977, 2000. PubMed: 10706684
61264: Barker K, et al. 3B11细胞Immortal and mortal clonal lymphocyte lines from channel catfish: comparison of telomere length, telomerase activity, tumor suppressor and heat shock protein expression. Dev. Comp. Immunol. 26: 45-51, 2002. PubMed: 11687262
61266: Miller N, et al. Functional and molecular characterization of teleost leukocytes. Immunol. Rev. 166: 187-197, 1998. PubMed: 9914913
