RMC细胞
器官来源： 肾脏
数量： 大量
生长状态： 贴壁生长
品系： Sprague-Dawley
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 褐鼠
年限： 3 months
ATCC Number： CRL-2573™
Designations: RMC
RMC细胞Depositors: M Ailenberg, M Silverman
Biosafety Level: 2 [Cells contain SV40 viral sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus deposited as rat
Morphology:
Source: Organ: kidney
Strain: Sprague-Dawley
Cellular Products: desmin; vimentin
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Age: 3 months
Gender: male
Comments: Rat mesangial cell at passage 8 were immortalized with pSV3-Neo and maintained in the presence of G418.
The cells express normal genes of the wild type mesangial cells; they are positive for desmin and vimentin and negative for cytokeratin 8.
Mesangial cells are crucial for kidney function and are used extensively in kidney research.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.4 mg/ml G418, 85%; fetal bovine serum, 15%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: RMC细胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37�C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: RMC细胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
