SJK-287-38细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
数量： 大量
生长状态： 悬浮生长
ATCC Number： CRL-1644™
细胞形态： **样
**类型： IgG1
运输方式： 冻存运输
Designations: SJK-287-38
Depositors: D Korn
Isotype: IgG1
SJK-287-38细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human DNA polymerase alpha
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. SJK-287-38细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: H-2db
Comments: Mice were immunized with fraction VIII human DNA polymerase alpha.
Spleen cells were fused with NS-1 myeloma cells.
The antibody neutralizes polymerase alpha activity, but does not react with DNA polymerases beta and gamma.
Tested and found negative for ectromelia virus (mousepox).
Formerly ATCC TIB-179.
Propagation: SJK-287-38细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 959: Hu SZ, et al. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. J. Biol. Chem. 257: 8386-8390, 1982. SJK-287-38细胞PubMed: 7085672
