3H5-1细胞
生长状态： 悬浮生长
数量： 大量
ATCC Number： HB-46™
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： **样
**类型： IgG1
Designations: 3H5-1
3H5-1细胞Depositors: MK Gentry
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against a type specific determinant on Dengue virus-2)
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Tumorigenic: Yes
Comments: Animals were immunized with dengue virus type 2 antigens from infected mouse brains (the prototype strain New Guinea C was used).
The antibody is type specific.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Antibodies should be prepared as ascites in pristane primed BALB/c mice.
Tested and found negative for ectromelia virus (mousepox).
Propagation: 3H5-1细胞ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 922: Henchal EA, et al. 3H5-1细胞Dengue virus-specific and Flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. Am. J. Trop. Med. Hyg. 31: 830-836, 1982. PubMed: 6285749
