39-S细胞
ATCC Number: HB-8180™
数量: 大量
细胞形态: **样
**类型: IgG2a
生长状态: 悬浮生长
运输方式: 冻存运输
是否是肿瘤细胞: 0
物种来源: 小鼠
Designations: 39-S
39-S细胞Depositors: The United States of America
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against herpes simplex virus type 1 (HSV-1) internal capsid protein 8 (ICP 8)
Permits/Forms: 39-S细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with HSV-1.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
The antibody is type specific for HSV-1 but does not neutralize the virus.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 85%; fetal bovine serum, 15% -OR- RPMI 1640 medium, 85%; fetal bovine serum, 15%
Subculturing: 39-S细胞Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1178: Showalter SD, et al. Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP 4. Infect. Immun. 34: 684-692, 1981. PubMed: 6277788
3544: Zweig M, et al. Monoclonal antibodies to herpes simplex virus type 1 polypeptides. 39-S细胞US Patent 4,572,896 dated Feb 25 1986