EL4.BU.1.OUAr.1.1细胞
生长状态: 悬浮生长
品系: C57BL/6N
ATCC Number: TIB-41™
相关**: **瘤
细胞类型: T**细胞
是否是肿瘤细胞: 0
物种来源: 小鼠
细胞形态: **样
EL4.BU.1.OUAr.1.1细胞运输方式: 冻存运输
数量: 大量
Designations: EL4.BU.1.OUAr.1.1
Depositors: R Hyman
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: C57BL/6N
Cell Type: EL4.BU.1.OUAr.1.1细胞T lymphocyte;
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Antigen Expression: H-2b; Thy-1.2
Comments: This line was derived from the EL4 cell line (see ATCC TIB-39).
The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine and 1 mM ouabain.
EL4.BU.1.OUAr.1.1细胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22247: Hyman R, Stallings V. EL4.BU.1.OUAr.1.1细胞Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.