3Pt12B8细胞
生长状态： 悬浮生长
ATCC Number： HB-8136™
是否是肿瘤细胞： 0
物种来源： 小鼠
数量： 大量
细胞形态： **样
**类型： IgM
3Pt12B8细胞运输方式： 冻存运输
Designations: 3Pt12B8
Depositors: Sidney Farber Cancer Institute
Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
3Pt12B8细胞Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against T12 (120000 dalton) antigen on human T cells (T lymphocyte); against CD6
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Tumorigenic: Yes
Comments: Animals were immunized with human T chronic lymphocytic leukemia cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody has been used to prevent graft versus host disease (GVHD).
Propagation: 3Pt12B8细胞ATCC complete growth medium: Modified Dulbecco's medium; horse serum, 10%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 3512: Reinherz EL, Schlossman SF. Monoclonal antibody. 3Pt12B8细胞US Patent 4,443,427 dated Apr 17 1984
