SH3细胞
数量： 大量
运输方式： 冻存运输
ATCC Number： HB-10744™
生长状态： 悬浮生长
细胞形态： **样
是否是肿瘤细胞： 0
物种来源： 小鼠
**类型： IgG2b
Designations: SH3
SH3细胞Depositors: Osiris Therapeutics, Inc.
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human mesenchymal stem cells
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Tumorigenic: Yes
Comments: Animals were immunized with human mesenchymal stem cells.
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
The antibody reacts with mesenchymal stem cells from bone marrow and some tissues of mesenchymal origin.
Propagation: ATCC complete growth medium: Modified Dulbecco's medium, 85%; fetal bovine serum, 15%
Temperature: 37.0°C
SH3细胞Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 22086: Caplan AI, Haynesworth SE. Human mesenchymal stem cells. US Patent 5,486,359 dated Jan 23 1996
49752: Haynesworth SE, et al. SH3细胞Cell surface antigens on human marrow-derived mesenchymal cells are detected by monoclonal antibodies. Bone 13: 69-80, 1992. PubMed: 1316137
