EL-NC-1S细胞
ATCC Number： HB-9647™
运输方式： 冻存运输
生长状态： 悬浮生长
是否是肿瘤细胞： 0
物种来源： 小鼠
数量： 大量
细胞形态： **样
**类型： IgG1
Designations: EL-NC-1S [anti NAP-1]
Depositors: National Cancer Institute/FCRF
EL-NC-1S细胞Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against neutrophil attractant/activation protein 1 (NAP-1, also known as interleukin 8 or IL-8 and monocyte derived neutrophil chemotactic factor, MDNCF)
Permits/Forms: EL-NC-1S细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with purified monocyte derived neutrophil chemotactic factor (MDNCF). [22353]
Spleen cells were fused with NS-1 mouse myeloma cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: EL-NC-1S细胞Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
References: 22353: Yoshimura T, et al. Three forms of monocyte-derived neutrophil chemotactic factor (MDNCF) distinguished by different lengths of the amino-terminal sequence. Mol. Immunol. 26: 87-93, 1989. PubMed: 2648135
23250: Sylvester I, et al. Secretion of neutrophil attractant/activation protein by lipopolysaccharide-stimulated lung macrophages determined by both enzyme-linked immunosorbent assay and N-terminal sequence analysis. Am. Rev. Respir. Dis. 141: 683-688, 1990. PubMed: 2178529
