El Don细胞
ATCC Number： CRL-1149™
相关**： 其他**
年限： 31 years
运输方式： 冻存运输
生长状态： 贴壁生长
器官来源： 皮肤
是否是肿瘤细胞： 0
物种来源： 人
El Don细胞数量： 大量
细胞形态： 成纤维样
Designations: El Don
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: El Don细胞Ehlers-Danlos syndrome, type VII (arthrocalasis type)
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Age: 31 years
Gender: female
Comments: The tissue donor suffered from type VII Ehlers-Danlos syndrome (arthrocalasis type), and was patient No. 2 in Lichtenstein, JR, et al. Science 182:298, 1973.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. El Don细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 7 to 9 days.
References: 22242: Lichtenstein JR, et al. El Don细胞Defect in conversion of procollagen to collagen in a form of Ehlers-Danlos syndrome. Science 182: 298-300, 1973. PubMed: 4742738
