SH-34细胞
运输方式： 冻存运输
ATCC Number： CRL-2405™
是否是肿瘤细胞： 0
物种来源： 小鼠
数量： 大量
生长状态： 悬浮生长
细胞形态： **样
**类型： IgM
SH-34细胞Designations: SH-34
Depositors: TJ Higgins
Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: SH-34细胞immunoglobulin; monoclonal antibody; against asialo-GM1
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Comments: Animals were immunized with the glycolipid asialo GM1 adsorbed to naked Salmonella.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for asialo-GM1. This antibody recognizes and kills by complement lysis cells known to express asialo GM1.
Most natural killer (NK) cells and cytotoxic T cells (CTL), as well as a proportion of other myeloid and lymphoid cells express asialo GM1.
The SH-34 antibody specifically lyses asialo GM1-expressing macrophages in the presence of complement and removes NK cells in vitro from spleen cell populations. SH-34细胞[38640]
This monoclonal antibody can be used for the study of murine and rat hematopoietic and lymphopoietic cell lineages.
Propagation: ATCC complete growth medium: Modified Dulbecco's medium, 95%; fetal bovine serum, 5%
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Preservation: Culture medium, 85%; fetal bovine serum, 10%; DMSO, 5%
Related Products: SH-34细胞recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 38639: Stout RD, et al. Evidence that expression of asialo-GM1 may be associated with cell activation. Correlation of asialo-GM1 expression with increased total cellular RNA and protein content in normal thymocyte and spleen cell populations. J. Immunol. 139: 2123-2129, 1987. PubMed: 2443562
38640: Solomon FR, Higgins TJ. A monoclonal antibody with reactivity to asialo GM1 and murine natural killer cells. Mol. Immunol. 24: 57-65, 1987. PubMed: 3614206
38642: Suttles J, et al. Flow cytometric analysis reveals the presence of asialo GM1 on the surface membrane of alloimmune cytotoxic T lymphocytes. J. Immunol. 136: 1586-1591, 1986. PubMed: 2936802
