36.5细胞
细胞形态： 上皮样
生长状态： 贴壁生长
ATCC Number： CRL-11116™
品系： 129/Sv+c/+p
数量： 大量
细胞类型： 其他细胞类型
器官来源： 胚胎
是否是肿瘤细胞： 0
物种来源： 小鼠
年限： embryo, blastocyst
36.5细胞运输方式： 冻存运输
Designations: 36.5
Depositors: Ontario Cancer Institute
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: epithelial
Source: Organ: embryo
Strain: 129/Sv+c/+p
Cell Type: pluripotent embryonic stem cell;
Permits/Forms:36.5细胞 In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: embryo, blastocyst
Comments: This line was derived by inserting a construct containing a disrupted murine Lyt-2 (CD8) gene.
The Lyt-2 genes was disrupted by insertion of a plasmid containing a neomycin resistance gene into the first exon of the Lyt-2 gene.
After selection for neomycin resistance and presence of Lyt-2 sequences upstream of the insertion, the 36.5 cell line was established.
This line has been used to produce mice deficient in expression of CD8.
The cells can be maintained in the undifferentiated state by frequent subculture on feeder layers of irradiated (12000 rads) or mitomycin C treated (0.01 mg/ml for 90 minutes) primary mouse embryonic fibroblasts or STO cells.
(see ATCC CRL-1503 or ATCC 56-X, irradiated STO cells).
Propagation: 36.5细胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium and rinse the monolayer with fresh 0.25% trypsin, 0.03% EDTA solution. Remove the trypsin and incubate at 37C until the cells detach (approximately 10 minutes). Add fresh medium, aspirate and dispense onto fresh feeder layer cultures.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
feeder layer cells:ATCC 56-X
References: 22100: Mak TW. 36.5细胞Mutant mouse lacking CD8 surface marker. US Patent 5,530,178 dated Jun 25 1996
