EL 1细胞
生长状态： 悬浮生长
器官来源： 脾
细胞形态： 单核细胞/巨噬细胞
是否是肿瘤细胞： 0
物种来源： 人
ATCC Number： CRL-9854™
数量： 大量
运输方式： 冻存运输
Designations: EL 1
EL 1细胞Depositors: E.I. du Pont de Nemours & Co.
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: monocyte/macrophage
Source: Organ: spleen
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Comments: EL 1细胞The EL 1 cell line was established from cryopreserved human spleen cells.
The cells tend to grow as single cells but may form small nonadherent clusters.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; calf serum, 10%
Temperature: 37.0°C
EL 1细胞Subculturing: Medium Renewal: Every 2 to 3 days (or as necessary to maintain the cell concentration between 2 X 10 exp5 and 1 X 10 exp6 cells/ml)
Cultures can be maintained by addition or replacement of fresh medium. Maintain the cell concentration between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
References: 26300: Collins GW, Largen MT. EL 1细胞Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995
