EJG细胞
细胞类型： 内皮细胞
器官来源： 肾上腺
是否是肿瘤细胞： 0
物种来源： 牛
生长状态： 贴壁生长
ATCC Number： CRL-8659™
数量： 大量
相关**： 正常
细胞形态： 内皮样
运输方式： 冻存运输
EJG细胞组织来源： capillary endothelium
Designations: EJG
Depositors: The United States of America
Biosafety Level: 2 [Cells contain Bovine Viral Diarrhea Virus (BVDV) ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Bos taurus
Morphology: endothelial
Source: Organ: adrenal gland
Tissue: capillary endothelium
Disease: normal
Cell Type: endothelial
Cellular Products: EJG细胞Factor VIII:C
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: The line was derived from collagenase dissociated bovine adrenal tissue.
The cellular morphology is that of capillary endothelium.
The cells form linearly oriented confluent cultures.
Recent tests for bovine viral diarrhea virus (BVDV) indicate that the line is producing both detectable BVDV antigens and infectious BVDV virions (J. Virol Methods 48:211-221, 1994).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: 2 to 3 times per week
Remove medium, rinse flask with fresh 0.25% trypsin, 0.1% EDTA and allow the flask to sit at room temperature until the cells detach (about 5 minutes). EJG细胞Add fresh medium, aspirate and dispense into new flasks.
Seed new flasks (75 sq. cm.) with 1 X 10 exp4 cells.
Doubling Time: 110 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 3866: Ornberg R, et al. Isolation and culture of adrenal medullary endothelial cells producing blood clotting factor VIII:C. US Patent 4,670,394 dated Jun 2 1987
22883: Bolin SR, et al. EJG细胞Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
