SFR3-DR5细胞
生长状态： 悬浮生长
是否是肿瘤细胞： 0
物种来源： 大鼠
数量： 大量
运输方式： 冻存运输
细胞形态： **样
**类型： IgG2b
ATCC Number： HB-151™
SFR3-DR5细胞Designations: SFR3-DR5
Depositors: SF Radka
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: SFR3-DR5细胞immunoglobulin; monoclonal antibody; against HLA DR5
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with purified DR antigen from obtained from the Swei human lymphoblastoid cell line.
Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. SFR3-DR5细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1001: Radka SF, et al. SFR3-DR5细胞SFR3-DR5, a monoclonal antibody with HLA-DR5 specificity. J. Immunol. 130: 1863-1866, 1983. PubMed: 6187822
