EIAV 12E8.1细胞
数量： 大量
细胞形态： **样
**类型： IgG1
ATCC Number： HB-8917™
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
运输方式： 冻存运输
EIAV 12E8.1细胞Designations: EIAV 12E8.1
Depositors: Fermenta Animal Health Company
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: EIAV 12E8.1细胞immunoglobulin; monoclonal antibody; against equine infectious anemia virus (EIA, EIAV) core antigen (p26)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with p26 prepared from the Wyoming strain of EIA virus.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Propagation: EIAV 12E8.1细胞ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 85%; fetal bovine serum, 15%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: DMEM, 70%; fetal bovine serum, 20%; DMSO, 10%
References: 3914: Matsushita T, et al. Method for the detection of equine infectious anemia and other retrovirus infections using a competitive enzyme-linked immunoabsorbent assay and reagents useful in the same. EIAV 12E8.1细胞US Patent 4,806,467 dated Feb 21 1989
