EHS细胞
数量： 大量
细胞形态： 成纤维样
是否是肿瘤细胞： 0
物种来源： 小鼠
品系： C57BL
运输方式： 冻存运输
组织来源： connective tissue
ATCC Number： CRL-2108™
相关**： 其他**
Designations: EHS
EHS细胞Depositors: H Kleinman
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Organism: Mus musculus
Morphology: fibroblast
Source: Tissue: connective tissue
Strain: C57BL
Disease: sarcoma
Cellular Products: laminin; collagen IV; entactin; heparin sulfate proteoglycan
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. EHS细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: EHS (Englebreth-Holm-Swarm) is a sarcoma carried as a transplantable tumor in C57BL mice.
It produces all the basement membrane components (laminin, collagen IV, entactin, and heparan sulfate proteoglycan).
The EHS sarcoma is a source of Matrigel, an extract that stimulates cell differentiation.
Matrigel is rich in laminin, growth factors, entactin/nidogen, type IV collagen, and heparan sulfate proteoglycan.
The EHS sarcoma is a rich source of both individual basement membrane components and Matrigel.
The ATCC stocks were established from tumors grown in C57BL/6J mice.
Propagation: EHS细胞ATCC complete growth medium: Duration: These cell are propagated in C57BL/6 mice.
Subculturing: Protocol: Harvest tumor from one mouse, mince and inject intramuscularly (over the hindlimb) into 3 to 10 C57BL/6 mice. Examine the mice daily for tumor progression, and harvest tumors (usually about 14 to 21 days after inoculation).
Preservation: Freeze medium: Ham's F12K medium, 80%; fetal bovine serum; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
References: 22608: Kleinman HK, et al. Basement membrane complexes with biological activity. Biochemistry 25: 312-318, 1986. PubMed: 2937447
22966: . . J. Tissue Culture Methods 16: 227-230, 1994.
23396: Timpl R, et al. EHS细胞Laminin--a glycoprotein from basement membranes. J. Biol. Chem. 254: 9933-9937, 1979. PubMed: 114518
