EH17a细胞
生长状态： 悬浮生长
运输方式： 冻存运输
细胞形态： **样
**类型： IgG1
数量： 大量
ATCC Number： CRL-2209™
是否是肿瘤细胞： 0
物种来源： 小鼠
EH17a细胞Designations: EH17a
Depositors: JD Ernst
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: EH17a细胞hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human annexin I
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with a mixture of Ca++ dependent phospholipid binding proteins isolated from human polymorphonuclear leukocytes.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with human, bovine and rat annexin I.
It does not inhibit annexin I mediated phospholipid vesicle aggregation.
Propagation: EH17a细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 23407: Ernst JD, et al. Identification of a domain that mediates vesicle aggregation reveals functional diversity of annexin repeats. J. Biol. Chem. 266: 6670-6673, 1991.EH17a细胞 PubMed: 1707872
