35.1细胞
数量： 大量
ATCC Number： HB-222™
运输方式： 冻存运输
细胞形态： **样
是否是肿瘤细胞： 0
物种来源： 小鼠
**类型： IgG2a
生长状态： 悬浮生长
Designations: 35.1
35.1细胞Depositors: PJ Martin
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human CD2
Permits/Forms: 35.1细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with human T lymphocytes that had been activated with phorbol myristic acid (PMA).
Spleen cells were fused with NSI/1 myeloma cells.
The antibody reacts with the human T cell surface protein Tp50 (CD2).
The antibody inhibits T cell proliferative responses and cytotoxicity, but does not inhibit antibody mediated cellular cytotoxicity.
Propagation: 35.1细胞ATCC complete growth medium: RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
References: 1792: Martin PJ, et al. Identification and functional characterization of two distinct epitopes on the human T cell surface protein Tp50. J. Immunol. 131: 180-185, 1983. 35.1细胞PubMed: 6190907
