SF1-1.1.10细胞
运输方式： 冻存运输
数量： 大量
ATCC Number： HB-159™
生长状态： 悬浮生长
细胞形态： **样
**类型： IgG2a
是否是肿瘤细胞： 0
物种来源： 小鼠
Designations: SF1-1.1.10
SF1-1.1.10细胞Depositors: AM Stall
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: SF1-1.1.10细胞immunoglobulin; monoclonal antibody; against H-2 K d
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Comments: Animals were immunized with spleen cells from BALB/c mice.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody does not react with the b, f, k, p, q, s or v H-2 haplotypes.
SF1-1.1.10细胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. SF1-1.1.10细胞Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
