Sf 1 Ep (NBL-11)细胞
ATCC Number： CCL-68™
运输方式： 冻存运输
数量： 大量
生长状态： 贴壁生长
年限： *****
是否是肿瘤细胞： 0
物种来源： 其他
器官来源： 皮肤
细胞形态： 上皮样
Sf 1 Ep (NBL-11)细胞组织来源： epidermis
Designations: Sf 1 Ep (NBL-11)
Depositors: AJ Kniazeff, WA Nelson-Rees, RB Owens
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Sylvilagus floridanus
Morphology: epithelial
Sf 1 Ep (NBL-11)细胞Source: Organ: skin
Tissue: epidermis
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance: poliovirus 2; coxsackievirus A9, B5; adenovirus 5
Age: *****
Gender: male
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: Sf 1 Ep (NBL-11)细胞37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium Sf 1 Ep (NBL-11)细胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
