24IA2E2E10D5 [24IA2]细胞
ATCC Number： HB-8049™
运输方式： 冻存运输
生长状态： 悬浮生长
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： **样
**类型： IgG
数量： 大量
Designations: 24IA2E2E10D5 [24IA2]
24IA2E2E10D5 [24IA2]细胞Depositors: Regents of the University of California
Isotype: IgG
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against the p27 protein of Feline Leukemia virus (FeLV)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 24IA2E2E10D5 [24IA2]细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with purified FeLV p27.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the A, B, C, AB and ABC strains of FeLV.
The antibody does not react with RD114 virus or AKR Murine Leukemia virus (MuLV).
Propagation: 24IA2E2E10D5 [24IA2]细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 3873: Lutz H. Hybridomas producing monoclonal antibodies specific for FeLV p27.24IA2E2E10D5 [24IA2]细胞 US Patent 4,713,325 dated Dec 15 1987
