22D1细胞
细胞形态： **样
**类型： hamster, Armenian IgG
生长状态： 悬浮生长
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
运输方式： 冻存运输
ATCC Number： CRL-2841™
Designations: 22D1
Depositors: CG Park, RM Steinman
22D1细胞Isotype: hamster, Armenian IgG
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybrid
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 22D1细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: May, 2003
Comments: Animals were immunized with mouse SIGN-R1 protein and their spleen cells were fused with mouse myeloma cells. SIGN-R1 is a C-type lectin. The antibody selectively binds to mouse SIGN-R1 but not to cells transfected with other lectins. The antibody is useful for FACS and immunocytochemistry assays.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: 22D1细胞Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(4) to 4 X 10(4) viable cells/ml.
Interval: Subculture when cells reach a concentration of 8 X 10(5) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 12 hours
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
Cell culture tested DMSO:ATCC 4-X
Erythrosin B vital stain solution:ATCC 30-2404
Trypan Blue vital stain solution:ATCC 30-2402
References: 89154: Kang YS, et al. 22D1细胞The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen. Proc. Natl. Acad. Sci. USA 101: 215-220, 2004. PubMed: 14694198
