RDP 45/20细胞
ATCC Number： TIB-98™
细胞形态： **样
**类型： IgM
是否是肿瘤细胞： 0
物种来源： 小鼠
运输方式： 冻存运输
生长状态： 悬浮生长
数量： 大量
Designations: RDP 45/20
RDP 45/20细胞Depositors: WC Raschke
Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; RDP 45/20细胞monoclonal antibody; against the Igh-1b allotypic determinant on mouse IgG2a
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Comments: Animals were immunized with CBPC-101 myeloma protein [IgG2a (Ig-1b)].
Spleen cells were fused with the MPC-11 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: RDP 45/20细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
References: 2127: DiPauli R, Raschke WC. RDP 45/20细胞A hybridoma secreting IgM anti-IgIb allotype. Curr. Top. Microbiol. Immunol. 81: 37-39, 1978. PubMed: 567562
