E6(2)2细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
运输方式： 冻存运输
ATCC Number： HB-8172™
数量： 大量
细胞形态： **样
**类型： IgG1
Designations: E6(2)2
E6(2)2细胞Depositors: Research Corporation
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; E6(2)2细胞monoclonal antibody; against vitamin B6
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with phosphopyridoxyl reduced DNA binding proteins.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with B6 vitamers.
It binds to both pyridoxine and pyridoxamine with similar affinities.
E6(2)2细胞Since the product binds an essential nutrient, the medium must be changed frequently or the cells will die.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, and 0.1 mM non-essential amino acids, 70%; NCTC 109 medium, 10%; fetal bovine serum, 20%
Subculturing: Medium Renewal: 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: fetal bovine serum, 95%; DMSO, 5%
References: 21902: Cidlowski JA, Viceps-Madore D. E6(2)2细胞Monoclonal antibodies to vitamin B.sub.6 and immunoassay method. US Patent 4,465,776 dated Aug 14 1984
