E5BB3IIA2细胞
细胞形态： **样
是否是肿瘤细胞： 0
物种来源： 小鼠
**类型： IgG2a; kappa light chain
生长状态： 悬浮生长
运输方式： 冻存运输
ATCC Number： HB-121™
数量： 大量
E5BB3IIA2细胞Designations: E5BB3IIA2
Depositors: RP Siraganian
Isotype: IgG2a; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: E5BB3IIA2细胞immunoglobulin; monoclonal antibody; against human IgE
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Comments: Animals were immunized with purified IgE myeloma protein.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody induces histamine release from human basophils.
E5BB3IIA2细胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 58009: Hook WA, et al. E5BB3IIA2细胞Detection of different antigenic sites on human IgE using monoclonal antibodies. Fed. Proc. 42: 713, 1983.
