26ic细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
ATCC Number： HB-246™
数量： 大量
运输方式： 冻存运输
生长状态： 悬浮生长
细胞形态： **样
**类型： IgG2b; kappa light chain
Designations: 26ic
26ic细胞Depositors: RF Todd
Isotype: IgG2b; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 26ic细胞immunoglobulin; monoclonal antibody; against human CD14
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Comments: Animals were immunized with cultured human peripheral blood monocytes.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with a nonfunctional domain of human CD14.
26ic细胞The binding of 26ic does not inhibit CD14 mediated activities, and is useful for detecting CD14 expression by immunofluorescence and or immunocytochemical methods.
Propagation: ATCC complete growth medium: Modified Dulbecco's medium, 90%; heat-inactivated gamma globulin free horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 22487: Todd RF, et al. Structural analysis of differentiation antigens Mo1 and Mo2 on human monocytes. Hybridoma 1: 329-337, 1982. PubMed: 6208133
23506: Wright SD, et al. 26ic细胞CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 249: 1431-1433, 1990. PubMed: 1698311
