E.H. IV (Elaine IV)细胞
运输方式: 冻存运输
细胞类型: B**细胞
是否是肿瘤细胞: 0
物种来源: 人
生长状态: 悬浮生长
ATCC Number: CCL-104™
相关**: 其他**
细胞形态: **样
数量: 大量
E.H. IV (Elaine IV)细胞器官来源: 外周血
年限: 20 years
Designations: E.H. IV (Elaine IV)
Depositors: W Henle
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Organ: peripheral blood
Disease: infectious mononucleosis
Cell Type: E.H. IV (Elaine IV)细胞B lymphoblast;
Cellular Products: Epstein-Barr virus (EBV)
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Virus Resistance: partially resistant to poliovirus and vesicular stomatitis viruses
Isoenzymes: G6PD, B
Age: 20 years
Gender: female
Ethnicity: Caucasian
Comments: The cells carry and produce Epstein-Barr virus (EBV).
EBNA positive.
Biological Safety CLASS II.
Propagation:E.H. IV (Elaine IV)细胞 ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
Cultures can be maintained by addition of fresh medium or by replacement of medium by sedimentation. Establish new cultures by seeding in fresh medium at 5 X 10 exp5 viable cells/ml.
References: 21415: Henle G, et al. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc. Natl. Acad. Sci. USA 59: 94-101, 1968. PubMed: 5242134
26274: Niederman JC, et al. Infectious mononucleosis. Clinical manifestations in relation to EB virus antibodies. E.H. IV (Elaine IV)细胞JAMA 203: 205-209, 1968. PubMed: 4864269