RAW 8.1细胞
生长状态： 悬浮生长
品系： BALB/c
细胞类型： B**细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： **样
ATCC Number： TIB-50™
相关**： **瘤
RAW 8.1细胞数量： 大量
运输方式： 冻存运输
器官来源： 脾
Designations: RAW 8.1
Depositors: P Ralph
Biosafety Level: 2
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Organ: RAW 8.1细胞spleen
Strain: BALB/c
Disease: lymphoma
Cell Type: B lymphocyte;
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Comments: RAW 8.1 was established from a tumor induced by Abelson murine leukemia virus.
Growth of this line is inhibited by 5 mcg/ml LPS.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. RAW 8.1细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1137: Ralph P, Nakoinz I. Lipopolysaccharides inhibit lymphosarcoma cells of bone marrow origin. Nature 249: 49-51, 1974. PubMed: 4208429
2104: Ralph P, et al. Lymphosarcoma cell growth is selectively inhibited by B lymphocyte mitogens: LPS, dextran sulfate and PPD. Biochem. Biophys. Res. Commun. 61: 1268-1275, 1974. RAW 8.1细胞PubMed: 4616699
