E (Ehrlich-Lettre ascites)细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
运输方式： 冻存运输
生长状态： 贴壁生长
ATCC Number： CCL-77™
数量： 大量
E (Ehrlich-Lettre ascites)细胞细胞形态： 上皮样
Designations: E (Ehrlich-Lettre ascites)
Depositors: CW Boone
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: epithelial
Permits/Forms: E (Ehrlich-Lettre ascites)细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance: poliovirus 3
Comments: Tested and found negative for ectromelia virus (mousepox).
E (Ehrlich-Lettre ascites)细胞Propagation: ATCC complete growth medium: NCTC 135 medium, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Subcultures can be prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Alternatively, a standard trypsinization procedure can be used.
References: 22170: Boone C, et al. Characterization of an in vitro strain of Ehrlich-Lettre ascites carcinoma subjected to many periodic mouse passages. J. Natl. Cancer Inst. 34: 725-740, 1965. E (Ehrlich-Lettre ascites)细胞PubMed: 5829538
