Sarcoma 180细胞
品系： Swiss Webster
ATCC Number： TIB-66™
相关**： 其他**
生长状态： 悬浮生长
数量： 大量
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 小鼠
Designations: Sarcoma 180
Sarcoma 180细胞Depositors: M Cohn
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology:
Source: Strain: Swiss Webster
Disease: sarcoma
Derived from metastatic site: ascites
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Tumorigenic: Yes
Comments: Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Sarcoma 180细胞Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 32373: Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260
53560: Dunham LJ, Stewart HL. Sarcoma 180细胞A survey of transplantable and transmissible animal tumors. J. Natl. Cancer Inst. 13: 1299-1377, 1953. PubMed: 13035452
