Sal Mat细胞
运输方式： 冻存运输
器官来源： 皮肤
数量： 大量
是否是肿瘤细胞： 0
物种来源： 人
ATCC Number： CRL-1110™
相关**： 其他**
年限： 14 years
细胞形态： 成纤维样
生长状态： 贴壁生长
Designations: Sal Mat
Sal Mat细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: Lesch-Nyhan syndrome
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Sal Mat细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 14 years
Gender: male
Comments: The tissue donor suffered from Lesch-Nyhan syndrome.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Sal Mat细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Sal Mat细胞Subculture every 5 to 7 days.
