260-33C4细胞
细胞形态： **样
**类型： IgM
数量： 大量
生长状态： 悬浮生长
品系： 129GIX+
是否是肿瘤细胞： 0
物种来源： 小鼠
年限： *****
运输方式： 冻存运输
260-33C4细胞器官来源： 脾
ATCC Number： CRL-2667™
Designations: 260-33C4
Depositors: National Cancer Institute
Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Strain: 129GIX+
Organ: spleen
Cell Type: 260-33C4细胞hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against a RAF 1 synthetic oncogene peptide
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: *****
Comments: Spleen cells were fused with Sp2/0 myeloma cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: 260-33C4细胞Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
260-33C4细胞Storage temperature: liquid nitrogen vapor phase
