S5细胞
ATCC Number： HB-9255™
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： **样
**类型： IgG1
生长状态： 悬浮生长
数量： 大量
Designations: S5
S5细胞Depositors: Sloan-Kettering Institute for Cancer Research
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against a human astrocytoma cell line; gp120 glycoprotein
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Comments: Animals were immunized with the SK-MG-17 human astrocytoma cell line.
The antibody reacts with a glycoprotein (gp120) of relative molecular weight 120000 daltons.
The antigen is expressed on cultured human fibroblasts and sarcomas.
In normal tissues, S5 antigen expression is limited to visceral smooth muscle.
In a survey of tumors, S5 staining was limited to a subset of leiomyosarcomas and the staining was generally weaker than that with normal smooth muscle.
Propagation: S5细胞ATCC complete growth medium: RPMI 1640 medium with non-essential amino acids, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium, 90%; DMSO, 10%
References: 21984: Rettig WJ, et al. Cell-surface glycoproteins of human sarcomas. US Patent 5,059,523 dated Oct 22 1991
23062: Rettig WJ, et al. Differential expression of cell surface antigens and glial fibrillary acidic protein in human astrocytoma subsets. Cancer Res. 46: 6406-6412, 1986. S5细胞PubMed: 2877731
