201-45E9细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： **样
**类型： IgG1 and IgG2b
ATCC Number： CRL-2670™
运输方式： 冻存运输
生长状态： 悬浮生长
数量： 大量
Designations: 201-45E9
Depositors: National Cancer Institute
201-45E9细胞Isotype: IgG1 and IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against a v-src synthetic oncogene peptide
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Comments: 201-45E9细胞Animals were immunized with a synthetic polypeptide with the sequence LMCQCWRKDPEERPTF corresponding to peptides 494 to 509 of the v-src oncogene (formerly 273 to 284).
Spleen cells were fused with Sp2/0 myeloma cells.
In ELISA testing, the antibody cross reacts with the homologous region of FES, v-mos, erb B, v-src, FGR, yes, mil, FMS, abl, ros, lck, and MET. Weak activity was detected for PKS and fps.
Binding was not detected for c-mos, MOS, SRC, ros, rel, FMS, HIR, TRK, sea, and kit. In immunoblot testing, a protein of approximately 40 kd is detected in fetal brain.
The IgG1 reactivity of the antibody with the v-src peptide (494 to 509) was confirmed by ELISA.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: 201-45E9细胞Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium 201-45E9细胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
