S49.1TB.2细胞
生长状态： 悬浮生长
细胞类型： T**细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
ATCC Number： TIB-30™
相关**： **瘤
运输方式： 冻存运输
品系： BALB/c
细胞形态： **样
S49.1TB.2细胞数量： 大量
Designations: S49.1TB.2
Depositors: A Harris
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: BALB/c
Cell Type: S49.1TB.2细胞T lymphocyte;
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Antigen Expression: H-2d; Thy-1.2; TL
Comments: This line was derived from S49.1 (ATCC TIB-28) by selecting for resistance to 10 mM thymidine and 0.1 mM 5-bromo-2'-deoxyuridine.
Like the parent line, the cells are sensitive to 0.001 mM cortisol (hydrocortisone), 5 mcg/ml phytohemagglutinin and 0.01 mM 6-thioguanine.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing: S49.1TB.2细胞Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1130: Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928
22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579
22241: Schiller JG, et al. S49.1TB.2细胞Glucocorticoid receptors in lymphoma cells in culture: relationship to glucocorticoid killing activity. Science 171: 189-191, 1971. PubMed: 4395230
