RA3-2C2/1细胞
细胞形态： **样
**类型： IgM
数量： 大量
运输方式： 冻存运输
生长状态： 悬浮生长
ATCC Number： TIB-145™
是否是肿瘤细胞： 0
物种来源： 大鼠
Designations: RA3-2C2/1
RA3-2C2/1细胞Depositors: RL Coffman
Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse B cell antigen (p50, 50000 dalton)
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Comments: Animals were immunized with the mouse cell line RAW 112.
Spleen cells were fused S194/5.XX0.BU.1 myeloma cells.
The antibody reacts with an antigen on normal B lymphocytes, a fraction of sIg- bone marrow cells and most plasma cells.
It precipitates a 50000 dalton (p50) molecule from bone marrow, spleen, thymus and Abelson leukemia virus transformed cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. RA3-2C2/1细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
References: 925: Coffman RL, Weissman IL. A monoclonal antibody that recognizes B cells and B cell precursors in mice. J. Exp. Med. 153: 269-279, 1981. PubMed: 6787164
926: Rotter V, et al. RA3-2C2/1细胞Abelson murine leukemia virus-induced tumors elicit antibodies against a host cell protein, P50. J. Virol. 36: 547-555, 1980. PubMed: 6159484
