S49.1G.3 PHA.100/0细胞
细胞类型： T**细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
品系： BALB/c
运输方式： 冻存运输
数量： 大量
细胞形态： **样
ATCC Number： TIB-35™
相关**： **瘤
S49.1G.3 PHA.100/0细胞生长状态： 悬浮生长
Designations: S49.1G.3 PHA.100/0 (replaces S49.1G3 PHA.12P)
Depositors: P Ralph
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: BALB/c
Cell Type: S49.1G.3 PHA.100/0细胞T lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: H-2d; Thy-1.2; TL
Comments: This variant of S49.1G.3 (ATCC TIB-34) was selected for resistance to 5 mcg/ml phytohemagglutinin.
The cells are also resistant to 6-thioguanine and sensitive to thymidine, and hydrocortisone.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. S49.1G.3 PHA.100/0细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1130: Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928
22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. S49.1G.3 PHA.100/0细胞Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579
