UMNSAH/DF-1细胞
细胞类型： 其他细胞类型
是否是肿瘤细胞： 0
物种来源： 其他
生长状态： 贴壁生长
年限： 10 days gestation
数量： 大量
细胞形态： 成纤维样
运输方式： 冻存运输
品系： East Lansing Line (ELL-0)
UMNSAH/DF-1细胞器官来源： 胚胎
ATCC Number： CRL-12203™
Designations: UMNSAH/DF-1
Depositors: Regents of the University of Minnesota
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Gallus gallus deposited as chicken
Morphology: fibroblast
Source: Organ: embryo
Strain: UMNSAH/DF-1细胞East Lansing Line (ELL-0)
Cell Type: fibroblastspontaneously transformed
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Virus Susceptibility: Meleagrid herpesvirus 1 (Herpes Virus of Turkey), Fowlpox Virus, reovirus, Avian Sarcoma Leukemia Virus and Rous Sarcoma Virus
Tumorigenic: No
Reverse Transcript: negative
Cytogenetic Analysis: Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22%
Age: 10 days gestation
Comments: UMNSAH/DF-1细胞UMNSAH/DF-1 is a spontaneously immortalized chicken cell line derived from 10 day old East Lansing Line (ELL-0) eggs.
Primary chicken embryonic fibroblasts were dissociated and grown in culture; the fibroblasts were passaged until they began to senesce; the cells were concentrated during cell senescence to maintain about 30% to about 60% culture confluence;
foci of non-senescent cells were identified and grown for greater than 30 passages.
No clonal proliferation was observed in soft agar cultures, indicating that these cells were immortalized but not transformed.
The cells are useful as substrates for virus propagation, recombinant protein expression and recombinant virus production.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 39.0°C; (Max. 40.0 °C, Min. 38.0°C)
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: UMNSAH/DF-1细胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 39�C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 39�C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 43260: Foster DN, Foster LK. Immortalized cell lines for virus growth. US Patent 5,672,485 dated Sep 30 1997
