R6.5.D6.E9.B2 [R6-5-D6]细胞
器官来源： 脾
生长状态： 悬浮生长
ATCC Number： HB-9580™
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： **样
**类型： mouse IgG2a kappa
年限： *****
运输方式： 冻存运输
R6.5.D6.E9.B2 [R6-5-D6]细胞品系： BALB/c
数量： 大量
Designations: R6.5.D6.E9.B2 [R6-5-D6]
Depositors: Dana Farber Cancer Inst.
Isotype: mouse IgG2a kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Organ: spleen
Strain: R6.5.D6.E9.B2 [R6-5-D6]细胞BALB/c
Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human intracellular adhesion molecule 1 (ICAM-1)
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Age: *****
Comments: Animals were immunized with the EBV transformed lymphoblast cell line, JY.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody binds to ICAM-1 and inhibits the interaction of ICAM-1 with leukocytes.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
R6.5.D6.E9.B2 [R6-5-D6]细胞Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 6 X 10(5) cells/ml.
Interval: Maintain cultures at a cell concentration between 3 X 10(4) and 5 X 10(5) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with extra 10% FBS and 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 12 hours
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22051: Springer TA, et al. R6.5.D6.E9.B2 [R6-5-D6]细胞Intercellular adhesion molecules, and their binding ligands. US Patent 5,284,931 dated Feb 8 1994
